CPT Tube for Extracting the Mononuclear Cells in Vitro

Usage
Purposes: mainly used to extract the mononuclear cells in vitro.

The internal structure
Upper: Anticoagulants or Anticoagulants Buffer.
Middle: Thixotropic Separating Gel.
Bottom: Mononuclear Cell Separation Medium.

Significance
Mononuclear cells includes lymphocytes and monocytes. At present, the research and application of mononuclear cells gain more attention. This product simplifies the clinical or laboratory steps to improve the clinical efficiency of the extraction of mononuclear cells, and lays the foundation for further extraction, purification, and transformation activation of the lymphocytes and for treatment of cancer.

1)The HBH CPT Tube should be at room temperature (18-25ºC) and properly labeled for patient identification.
)2) Spray the CPT Vacutainer lightly with 70% isopropanol/ethanol, wipe the alcohol off.
3)3) Collect blood into CPT tubes.
4) After collection, store tube upright at room temperature until centrifugation. Blood samples should be centrifuged within two hours of blood collection for best results.
5) Centrifuge tube/blood sample at room temperature (18-25ºC) in a horizontal rotor (swing-out head) for a minimum of 20 minutes (up to 30 minutes) at 1500 to 1800 RCF.
6)NOTE: Ensure that the CPT tubes have enough clearance from the rotor head in order to become fully horizontal during centrifugation. Failure to do so will cause severe damage to the CPT tubes and the centrifuge.